Spheroides: Measurement of Cell Aggregates after Dissociation
As a system for cell cultures, spheroids reflect the physiological organization of tissues better than traditional 2D cultures .
However, for analyzing the cells in a flow cytometer, dissociation into single cells is required.
U. Grässer et al. recently evaluated methods for dissociation of Spheroids. To quantify remaining cell aggregates, and for the determination of cell diameters, CASY was employed. (Annals of Anatomy – Anatomical indicators volume 216, March 2018, Pages 1-8; DOI: https://doi.org/10.1016/j.aanat.2017.10.002)
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